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HMP Research Precision Medicine Hub

Microbiome

16S Metagenomics

Microbial composition, species by species.

16S rRNA sequencing to describe the composition and diversity of microbial communities in a sample.

8–12 business days
Illustration — 16S Metagenomics

Overview

16S sequencing characterizes bacterial diversity in clinical or environmental samples. It supports group comparisons, dysbiosis monitoring and treatment-effect studies.

Analysis includes demultiplexing, filtering, ASV/OTU clustering, taxonomic assignment and alpha/beta diversity indices.

Method & technologies

  • V3–V4 amplification
  • MiSeq 2×300 bp sequencing
  • DADA2 / QIIME2
  • SILVA assignment

Deliverables

  • ASV abundance table
  • Alpha/beta diversity analysis
  • PCoA and barplot figures
  • PDF report