Expression
RNA Sequencing (RNA-seq)
What the genome actually expresses.
mRNA sequencing for differential expression, fusions and transcriptional profile characterization.
12–18 business days
Overview
RNA-seq measures the expression of all transcripts in a sample. It reveals differentially expressed genes, isoforms, gene fusions and transcriptional signatures.
Analyses include read QC, transcript quantification, differential analysis and functional enrichment.
Method & technologies
- Total RNA or polyA
- Illumina paired-end
- Salmon / DESeq2 quantification
- GO/KEGG enrichment
Deliverables
- Normalized expression matrix
- Differential gene list
- Figures and PDF report
- Reproducible scripts